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KMID : 0359319930330030547
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Korean Journal of Veterinary Research
1993 Volume.33 No. 3 p.547 ~ p.554
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Production of cloning animals by fresh and frozen - thawed nuclear transfer embryos Ⅱ
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Abstract
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This study was carried out to investigate the best condition for in vitro and in vivo culture after freezing and thawing of nuclear transplant 2 cell embryos.
When nuclear transplant embryos were submitted to electrofusion, the significantly higher fusion rates of 2 cell donor nuclei were achieved at the electric field strength of DC 1.5 ㎸/㎝ for 100 and 150 μ sec, DC 2.0 ㎸/㎝ for 100 and 150 μsec than DC 1.0 ㎸/㎝ for 100 and 150 /μsec(p<0.01). The significantly higher fusion rates of 4 cell donor nuclei were achievecl at DC 2.0 ㎸/㎝ for 100 and 150 μ sec than DC 1.0 ㎸/㎝ for 100 and 150 μsec(p<0.01). The fusion rates in 8 cell donor nuclei were 94.2∼99.3%.
The developmental potency to blastocyst in 2 cell donor nuclei was significantly higher in DC 2.0 ㎸/㎝ for 150 μsec treated group(p<0.01). The significantly higher developmental potency to blastocysl in 4 cell donor nuclei were achieved at the electric field strength of DC 2.0 ㎸/㎝ for 150 u sec than DC 1.5 ㎸/㎝ for 100 and 150 μsec, DC 2.0 ㎸/㎝ for 100 μsec treated group(p<0.01). The develop mental potency to blastocyst in 8 cell donor nuclei was significantly higher in DC 2.0 ㎸/㎝ for 100/μsec treated group(p<0.01). The developmental potency to blastocyst after nuclear transplantation was significantly higher in 2 cell donor nuclei than in 8 cell donor nuclei(p<0.01).
When the recovered embryos in normal morphology were cultured in vitro, there were no significant differences in the developmental potency to blastocyst between the freezing methods and the concentrations of cryoprotectant(p<0.01). The production rates of offspring after transfer of nuclear transplant embryos to recipient mouse were no significant difference in 2, 4 and 8 cell donor nuclei.
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KEYWORD
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